ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of effective parts of Zingiber officinal (EPZ) on the adhesion of ECV-304 cells with monocytes cultivated in vitro and on the expression of monocyte chemotactic protein-1 (MCP-1) and adhesion molecules.</p><p><b>METHOD</b>The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2). Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR. MCP-1 protein expression were detected by ELISA.</p><p><b>RESULT</b>EPZ could decrease the adhesion of monocytes with ECV-304 cells obviously. Meanwhile it could diminish the expression of ICAM-1, VCAM-1 and MCP-1 in injured ECV-304 cells.</p><p><b>CONCLUSION</b>EPZ could inhibit H2O2-induced ICAM-1, VCAM-1 and MCP-1 expression in ECV-304 and could inhibit the adherence of monocytes to endothelial cell, which may result in the protect effect in endothelial cells.</p>
Subject(s)
Animals , Humans , Rats , Cell Adhesion , Cell Line , Cells, Cultured , Chemokine CCL2 , Genetics , Drugs, Chinese Herbal , Pharmacology , Endothelial Cells , Cell Biology , Metabolism , Enzyme-Linked Immunosorbent Assay , Zingiber officinale , Chemistry , Hydrogen Peroxide , Pharmacology , Intercellular Adhesion Molecule-1 , Genetics , Monocytes , Cell Biology , Metabolism , Plants, Medicinal , Chemistry , RNA, Messenger , Genetics , Random Allocation , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction , Vascular Cell Adhesion Molecule-1 , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To evaluate the germplasm of Rehmannia glutinosa on the basis of photosynthetic pigment contents (PPC).</p><p><b>METHOD</b>20 cultivars were planted on the same condition. On Oct. 23 and Sept. 25, 3 leaves per cultivar were collected on different plants, and 80 mg mesophyll was collected among upper lateral veins and was ground in 96% alcohol, and the supernatant was subjected to measure on a spectrophotometer (Angilent 8453).</p><p><b>RESULT</b>The PPCs among cultivars were significantly different at a P < or = 0.01 level. The results of the measurements were similar. Chlolophyll a was the most abundant pigment, but varied to a great extent among different cultivars. 20 cultivars were divided into 9 homogeneous groups according to the contents of chlorophyll a by Duncan's multiple range test at P < or = 0.05. In addition, the content of chlorophyll a was closely related to leaf color. The cultivars with higher chlolophyll a had deep green leaves, and those with lower had yellow green or pale green leaves.</p><p><b>CONCLUSION</b>PPC was an inherent character and an important index for the germplasm evaluation of R. glutinosa.</p>